On March 5 - 8th, at 2 pm
Disciplina - EPIGENETIC MECHANISMS IN PSYCHIATRY AND APPROACHES
Speaker: Professor Richard Lee, Associate Professor in Psychiatry and Behavioral Sciences Johns Hopkins University, Baltimore, MA, USA
Coordinator: Mirian Hayashi, PhD, associate professor, Department of Pharmacology, Escola Paulista de Medicina - UNIFESP
Co-coordinator: Vitor Oliveira, PhD, associate professor, Department of Biophysics, Escola Paulista de Medicina - UNIFESP
Local: Anfiteatro INFAR (R 3 de maio 100)
Objetivos: O curso pretende proporcionar conhecimentos em candidate gene approaches and next Generation sequencing.
Ementa:
• Transcriptomic and epigenetic analysis of glucocorticoid treated neuronal cells and implication in repetitive elements and roles in antidepressant response;
• Impact of APOE deficiency on HPA axis function and cognition;
Speaker further information:
I received my B.S. in Biomedical Engineering from the Johns Hopkins University and a Ph.D. in Molecular Biology and Genetics at the Johns Hopkins School of Medicine. While doing my postdoc in the Department of Psychiatry, I became interested in elucidating the epigenetic mechanisms of the HPA axis and neuroendocrine hormones in the context of psychiatric disorders. Exposure to chronic stress is a risk factor for a number of diseases, including psychiatric disorders, and we were one of the first to demonstrate that stress hormone or glucocorticoids can directly alter DNA methylation in vivo. We employ both candidate loci and genome-wide approaches to identify target genes and pathways that are epigenetically affected by stress and glucocorticoids. I have designed over a hundred pyrosequencing assays for determination of DNA methylation in different species (human, mouse, rat, monkey) for my research program and that of others. I have also designed and implemented both microarray and sequencing based platforms for genome-wide DNA methylation projects, including the CHARM (Comprehensive High-throughput Arrays for Relative Methylation) platform and the SureSelect Methyl-Seq Target Enrichment System for the rodent genome. In addition, I have performed chromatin immunoprecipitation, in vitro reporter assays, electromobility shift assays, and chromosome conformation capture to demonstrate the functional role of epigenetic modifications on gene expression.
Specific areas of research (see also: Richard Sang Un Lee, Ph.D.)