Publications in the last 5 years

Infection by zika virus increase angiotensin I-converting enzyme activity in mouse brain

Oyadomari WY, Christoff RR, Nani JV, Rabello T, Oliveira V, Higa LM, Garcez PP, Hayashi MAF. Infection by zika virus increase angiotensin I-converting enzyme activity in mouse brain. Biochimie. 2024 Jul 2:S0300-9084(24)00160-3. doi: 10.1016/j.biochi.2024.07.001.

Sodium nitroprusside as an adjunctive treatment for schizophrenia reduces the Ndel1 oligopeptidase activity

Nani JV, Ushirohira JM, Bradshaw NJ, Machado-de-Sousa JP, Hallak JEC, Hayashi MAF. Sodium nitroprusside as an adjunctive treatment for schizophrenia reduces the Ndel1 oligopeptidase activity. Braz J Psychiatry. 2023 Nov 23. doi: 10.47626/1516-4446-2023-3315. doi: 10.47626/1516-4446-2023-3315. Online ahead of print.

Identification of an ex vivo inhibitor of the schizophrenia biomarker Ndel1 by high throughput screening

Identification of an ex vivo inhibitor of the schizophrenia biomarker Ndel1 by high throughput screening

Nani JV, Coelho C, Oyadomari WY, Santiago TC, Machado MM, Christoff RR, Garcez PP, Oliveira V, Würtele M, Hayashi MAF. EIdentification of an ex vivo inhibitor of the schizophrenia biomarker Ndel1 by high throughput screening. Biochem Pharmacol. 2023 Oct 9;217:115841. https://doi.org/10.1016/j.bcp.2023.115841

Evaluation of tumor growth remission in a murine model for subcutaneous solid tumors – Benefits of associating the antitumor agent crotamine with mesoporous nanosilica particles to achieve improved dosing frequency and efficacy.

Evaluation of tumor growth remission in a murine model for subcutaneous solid tumors – Benefits of associating the antitumor agent crotamine with mesoporous nanosilica particles to achieve improved dosing frequency and efficacy.

Oyadomari WY, Anthero GL, Silva MRA, Porta LC, Oliveira V, Reid PF, Sant'Anna OA, Alves WA, Nani JV, Hayashi MAF. Evaluation of tumor growth remission in a murine model for subcutaneous solid tumors – Benefits of associating the antitumor agent crotamine with mesoporous nanosilica particles to achieve improved dosing frequency and efficacy. Int. J. Pharm. 2023 646(5), 123420. https://doi.org/10.1016/j.ijpharm.2023.123420

Euthymic and depressed bipolar patients are characterized by different RNA editing patterns in blood

Hayashi MAF, Salvetat N, Cayzac C, Checa-Robles FJ, Dubuc B, Mereuze S, Nani JV, Molina F, Brietske E, Weissmann D. Euthymic and depressed bipolar patients are characterized by different RNA editing patterns in blood. Psychiatry Res. 2023 Aug 18. https://doi.org/10.1016/j.psychres.2023.115422.

Assessing the role of Ndel1 oligopeptidase activity in congenital Zika syndrome: Potential predictor of congenital syndrome endophenotype and treatment response

Christoff RR, Nani JV, Lessa G, Rabello T, Rossi AD, Krenn V, Higa LM, Tanuri A, Garcez PP, Hayashi MAF. Assessing the role of Ndel1 oligopeptidase activity in congenital Zika syndrome: Potential predictor of congenital syndrome endophenotype and treatment response. J Neurochem. 2023 Jul 27. doi: 10.1111/jnc.15918.

[18F]F-FDG and [11C]PK11195 PET imaging in the evaluation of brown adipose tissue - effects of cold and pharmacological stimuli and their association with crotamine intake in a male mouse model

Daniele de Paula Faria, Joana D'Arc Campeiro, Mara de Souza Junqueira, Caroline Cristiano Real, Fabio Luiz Navarro Marques, Mirian Akemi Furuie Hayashi, Marcelo Tatit Sapienza. [18F]F-FDG and [11C]PK11195 PET imaging in the evaluation of brown adipose tissue - effects of cold and pharmacological stimuli and their association with crotamine intake in a male mouse model. Nucl Med Biol. 2023 Jun 17;122-123:108362. doi: 10.1016/j.nucmedbio.2023.108362

Crotamine/siRNA Nanocomplexes for Functional Downregulation of Syndecan-1 in Renal Proximal Tubular Epithelial Cells

Joana D’Arc Campeiro, Wendy A. Dam, Mirian A. F. Hayashi, and Jacob van den Born. Crotamine/siRNA Nanocomplexes for Functional Downregulation of Syndecan-1 in Renal Proximal Tubular Epithelial Cells. Pharmaceutics 2023, 15, 1576. https://doi.org/10.3390/pharmaceutics15061576.

Prolyl oligopeptidase activity (POP) in early stage and medicated schizophrenia and in an animal model for schizophrenia study: in vivo effects of psychopharmacological substances on enzyme activity

Prolyl oligopeptidase activity (POP) in early stage and medicated schizophrenia and in an animal model for schizophrenia study: in vivo effects of psychopharmacological substances on enzyme activity

Lucas A.N. Marins, Benjamín Rodríguez, João V. Nania, Vitor Oliveira Ary Gadelha, Mirian A.F. Hayashi. Prolyl oligopeptidase activity (POP) in early stage and medicated schizophrenia and in an animal model for schizophrenia study: in vivo effects of psychopharmacological substances on enzyme activity. Brain Disorders 10 (2023) 100075. doi: 10.1016/j.dscb.2023.100075.

Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease

Boeynaems S, Ma XR, Yeong V, Ginell GM, Chen JH, Blum JA, Nakayama L, Sanyal A, Briner A, Van Haver D, Pauwels J, Ekman A, Schmidt HB, Sundararajan K, Porta L, Lasker K, Larabell C, Hayashi MAF, Kundaje A, Impens F, Obermeyer A, Holehouse AS, Gitler AD.Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease. bioRxiv. 2023 Mar 9:2023.03.09.531820. doi: 10.1101/2023.03.09.531820.

Angiotensin Converting Enzymes as druggable features of psychiatric and neurodegenerative disorders

¹H NMR Metabolomics and Lipidomics To Monitor Positive Responses in Children with Autism Spectrum Disorder Following a Guided Parental Intervention: A Pilot Study

Banny Silva Barbosa Correia, João Guilherme de Moraes Pontes, João Victor Silva Nani, Fabian Villalta, Natalia Cristina Mor, Daniela Bordini, Décio Brunoni, Helena Brentani, Jair Jesus Mari, Mirian A. F. Hayashi*, and Ljubica Tasic*. 1H NMR Metabolomics and Lipidomics To Monitor Positive Responses in Children with Autism Spectrum Disorder Following a Guided Parental Intervention: A Pilot Study. ACS Chem Neurosci. 2023 Mar 15;14(6):1137-1145. doi: 10.1021/acschemneuro.2c00735.

Doderlin: Isolation and Characterization of a Broad-Spectrum Antimicrobial Peptide from Lactobacillus acidophilus

Doderlin: Isolation and Characterization of a Broad-Spectrum Antimicrobial Peptide from Lactobacillus acidophilus

Bruna S.da Silva, AndreaDíaz-Roa, Erica S.Yamane, Mirian A.F.Hayashi, Pedro Ismael da Silva Junior. Doderlin: Isolation and Characterization of a Broad-Spectrum Antimicrobial Peptide from Lactobacillus acidophilus. Research in Microbiology 174 (2023) 103995. https://doi.org/10.1016/j.resmic.2022.103995.

Targeting Ca²⁺ and Mitochondrial Homeostasis by Antipsychotic Thioridazine in Leukemia Cells

Vivian W. R. Moraes, Vivian Matsukura dos Santos, Eloah R. Suarez, Leticia S. Ferraz, Rayssa M. Lopes, Giuliana P. Mognol, Joana D. Campeiro, João Agostinho Machado-Neto, Fabio D. Nascimento, Mirian A.F. Hayashi, Ivarne L.S. Tersariol, Donald D. Newmeyer, Tiago Rodrigues. Targeting Ca²⁺ and Mitochondrial Homeostasis by Antipsychotic Thioridazine in Leukemia Cells. Life 2022;12:1477. https://doi.org/10.3390/life12101477.

Brief reports unraveling the correlation among neurodevelopmental and inflammatory biomarkers in patients with chronic schizophrenia

Nani JV, Almeida PGC, Noto C, Bressan RA, Brietzke E, Hayashi MAF. BRIEF REPORTS UNRAVEILING THE CORRELATION AMONG NEURODEVELOPMENTAL AND INFLAMMATORY BIOMARKERS IN PATIENTS WITH CHRONIC SCHIZOPHRENIA. Nord J Psychiatry. 2022 Oct;76(7):559-564. doi: 10.1080/08039488.2021.2023217.

Crotamine trafficking dynamic in Plasmodium falciparum-infected erythrocytes: pharmacological inhibitors, parasite cycle and incubation time influences in uptake

Crotamine trafficking dynamic in Plasmodium falciparum-infected erythrocytes: pharmacological inhibitors, parasite cycle and incubation time influences in uptake

El Chamy Maluf S, Hayashi MAF, Campeiro JD, Oliveira EB, Gazarini ML, Carmona AK. Crotamine trafficking dynamic in Plasmodium falciparum-infected erythrocytes: pharmacological inhibitors, parasite cycle and incubation time influences in uptake. Toxicon. 202;208:47-52. doi: 10.1016/j.toxicon.2022.01.006.

Doderlin: Isolation and Characterization of a Broad-Spectrum Antimicrobial Peptide from Lactobacillus acidophilus

Doderlin: Isolation and Characterization of a Broad-Spectrum Antimicrobial Peptide from Lactobacillus acidophilus

Bruna S. da Silva, Andrea Diaz-Roa, Erica S. Yamane, Mirian A.F. Hayashi, Pedro Ismael Silva Junior. Doderlin: Isolation and Characterization of a Broad-Spectrum Antimicrobial Peptide from Lactobacillus acidophilus. bioRxiv 2022.01.19.476933; doi: https://doi.org/10.1101/2022.01.19.476933

Revisting the potential of South American rattlesnake Crotalus durissus terrificus toxins as therapeutic, theranostic and/or biotechnological agents

Revisting the potential of South American rattlesnake Crotalus durissus terrificus toxins as therapeutic, theranostic and/or biotechnological agents

Hayashi MAF, Campeiro JD, Yonamine CM. Revisting the potential of South American rattlesnake Crotalus durissus terrificus toxins as therapeutic, theranostic and/or biotechnological agents. Toxicon. Jan 30;206:1-13. doi: https://doi.org/10.1016/j.toxicon.2021.12.005

A native cell-penetrating peptide (CPP) from rattlesnake with therapeutic and theranostic properties

Porta LC, Campeiro JD, Hayashi MAF. A Native CPP from Rattlesnake with Therapeutic and Theranostic Properties. Methods Mol Biol. 2022;2383:91-104. doi: 10.1007/978-1-0716-1752-6_6.

Abstract
The cell-penetrating peptides (CPPs) are characterized by the ability of internalization into cells in vitro and in vivo, and the ability of these peptides can rely on a high content of positive charges, as it is the case of the native CPP crotamine. Crotamine is a polypeptide of about 42 amino acid residues with high content of basic residues as Arg and Lys. Although most of known CPPs are linear peptides, native the South American rattlesnake crotamine from the venom of a South American rattlesnake has a well-defined 3D structure stabilized by three disulfide bonds which guarantee the exposure of side chains of basic amino acids. This 3D structure also protects this amphipathic polypeptide from the degradation even if when administered by oral route, therefore, also protecting also the biological activities of crotamine. As several different biological properties of crotamine are dependent of cell penetration of crotamine, the methods mainly employed for analyzing crotamine properties as anthelminthic and antimalarial activities, antimicrobial and antitumor activities, with a unique selective cytotoxic property against actively proliferating cells, as tumor cells, were chosen based on crotamine its ability of internalization mediated by itsbased on positive charge. This native cationic polypeptide is also able to efficiently carry, with no need of covalent linkage with the cargo, genetic material into cells in vitroin vitro and in vivoin vivo, suggesting its use in gene therapy. Moreover, the possibility of decorating gold nanoparticles keeping the ability of transfecting cells was demonstrated. More recently, the ability of crotamine to interfere in animal metabolism, inducing browning of adipose tissue and increasing the energy expenditure, and its application in renal therapy was demonstrated. As crotamine also accumulates specifically in tumor cells in vivoin vivo, and the potential utility of crotamine as a theranostic agent was then suggested. Therefore, diverse methodologies employed for the characterization and exploration of the therapeutic applications of this promising native CPP for remediation of several pathogenic conditions are presented here.

Neuropeptidases in Psychiatric Disorders

A system biology approach based on metabolic biomarkers and protein-protein interactions for identifying pathways underlying Schizophrenia and Bipolar Disorder

Altaf-Ul-Amin M, Hirose K, Nani JV, Porta LC, Tasic L, Hossain SF, Huang M, Ono N, Hayashi MAF, Kanaya S. A system biology approach based on metabolic biomarkers and protein-protein interactions for identifying pathways underlying Schizophrenia and Bipolar Disorder. Sci Rep. 2021 Jul 14;11(1):14450. doi: 10.1038/s41598-021-93653-3.

Myotoxin a, Crotamine and Defensin Homologs in Reptile Venoms

Regulation of monoamine levels by typical and atypical antipsychotics in Caenorhabditis elegans mutant for Nuclear Distribution Element genes

Regulation of monoamine levels by typical and atypical antipsychotics in Caenorhabditis elegans mutant for Nuclear Distribution Element genes

D'Arc Campeiro J, Nani JV, Monte GG, Almeida PC, Mori MA, Hayashi MAF. Regulation of monoamine levels by typical and atypical antipsychotics in Caenorhabditis elegans mutant for Nuclear Distribution Element genes. Neurochem Int. 2021 Apr 17;147:105047. doi: 10.1016/j.neuint.2021.105047.

Effects of Psychostimulants and Antipsychotics on Serum Lipids in an Animal Model for Schizophrenia

Impact of Reck expression and promoter activity in neuronal in vitro differentiation

Evaluation of NDEL1 oligopeptidase activity in blood and brain in an animal model of schizophrenia: effects of psychostimulants and antipsychotics

Nani JV, Lee RS, Yonamine CM, Sant'Anna OA, Juliano MA, Gadelha A, Mari JJ, Hayashi MAF. Evaluation of NDEL1 oligopeptidase activity in blood and brain in an animal model of schizophrenia: effects of psychostimulants and antipsychotics. Sci Rep. 2020 Oct 28;10(1):18513. doi: 10.1038/s41598-020-75616-2.

Impairments in Peripheral Blood T Effector and T Regulatory Lymphocytes in Bipolar Disorder Are Associated with Staging of Illness and Anti-cytomegalovirus IgG Levels

MAES, M.. NANI, J.V., NOTO, C., RIZZO, L., HAYASHI, M. A. F., BRIETZKE, E. Impairments in Peripheral Blood T Effector and T Regulatory Lymphocytes in Bipolar Disorder Are Associated with Staging of Illness and Anti-cytomegalovirus IgG Levels. Mol Neurobiol. 2020 Jul 22:pyaa050. doi: 10.1007/s12035-020-02110-1.

Biophysical and pharmacological characterization of a full-length synthetic analog of the antitumor polypeptide crotamine

de Carvalho Porta L, Fadel V, D'Arc Campeiro J, Oliveira EB, Godinho RO, Hayashi MAF. Biophysical and pharmacological characterization of a full-length synthetic analog of the antitumor polypeptide crotamine. J Mol Med (Berl).2020 Nov;98(11):1561-1571. doi: 10.1007/s00109-020-01975-y.

A study in first-episode psychosis patients: does angiotensin I-converting enzyme (ACE) activity associated with genotype predict symptoms severity reductions after treatment with the atypical antipsychotic risperidone?

A study in first-episode psychosis patients: does angiotensin I-converting enzyme (ACE) activity associated with genotype predict symptoms severity reductions after treatment with the atypical antipsychotic risperidone?

Nani JV, Dal Mas C, Yonamine CM, Ota VK, Noto C, Belangero SI, Mari JJ, Bressan R, Cordeiro Q, Gadelha A, Hayashi MAF. A study in first-episode psychosis patients: does angiotensin I-converting enzyme (ACE) activity associated with genotype predict symptoms severity reductions after treatment with the atypical antipsychotic risperidone?. Int J Neuropsychopharmacol. 2020 Jul 22;23(11):721–30. doi: 10.1093/ijnp/pyaa050.

In vivo effects of the association of the psychoactive phenotiazine thioridazine on antitumor activity and hind limb paralysis induced by the native polypeptide crotamine

Porta LC, Campeiro JD, Papa GB, Oliveira EB, Godinho RO, Rodrigues T, Hayashi MAF. In vivo effects of the association of the psychoactive phenotiazine thioridazine on antitumor activity and hind limb paralysis induced by the native polypeptide crotamine. Toxicon 2020 Oct 15;185:64-71. https://doi.org/10.1016/j.toxicon.2020.06.017

Crotamine Cell-Penetrating Nanocarriers: Cancer-Targeting and Potential Biotechnological and/or Medical Applications

Hayashi MAF, Campeiro JD, Porta LC, Szychowski B, Alves WA, Oliveira EB, Kerkis I, Daniel MC, Karpel RL. Crotamine Cell-Penetrating Nanocarriers: Cancer-Targeting and Potential Biotechnological and/or Medical Applications. Methods Mol Biol. (Clifton, N.J.) Methods Mol Biol. 2020;2118:61-89. https://doi.org/10.1007/978-1-0716-0319-2_5

Decreased nuclear distribution nudE-like 1 enzyme activity in an animal model with dysfunctional disrupted-in-schizophrenia 1 signaling featuring aberrant neurodevelopment and amphetamine-supersensitivity

Decreased nuclear distribution nudE-like 1 enzyme activity in an animal model with dysfunctional disrupted-in-schizophrenia 1 signaling featuring aberrant neurodevelopment and amphetamine-supersensitivity

Nani JV, Fonseca MC, Engi SA, Perillo MG, Dias CS, Gazarini ML, Korth C, Cruz FC, Hayashi MA. Decreased nuclear distribution nudE-like 1 enzyme activity in an animal model with dysfunctional disrupted-in-schizophrenia 1 signaling featuring aberrant neurodevelopment and amphetamine-supersensitivity. J Psychopharmacol. (Oxford, England) 2020 Apr;34(4):467-477. https://doi.org/10.1177/0269881119897562

Neuropeptides and oligopeptidases in schizophrenia

Peripheral biomarkers allow differential diagnosis between schizophrenia and bipolar disorder

Tasic L, Larcerda ALT, Pontes JGM, da Costa TBBC, Nani JV, Martins LG, Santos LA, Nunes MFQ, Adelino MPM, Pedrini M, Cordeiro Q, Bachion de Santana F, Poppi RJ, Brietzke E, Hayashi MAF. Peripheral biomarkers allow differential diagnosis between schizophrenia and bipolar disorder. J Psychiatr Res. 2019 Dec;119:67-75. doi: 10.1016/j.jpsychires.2019.09.009. [Noticia agencia FAPESP]

Unraveiling the correlation among neurodevelopmental and inflammatory biomarkers in patients with chronic schizophrenia

Nani JV, Almeida PGC, Noto C, Bressan RA, Brietzke E, Hayashi MAF. Unraveiling the correlation among neurodevelopmental and inflammatory biomarkers in patients with chronic schizophrenia. NORDIC JOURNAL OF PSYCHIATRY (2022), doi: https://doi.org/10.1080/08039488.2021.2023217

Revisting the potential of South American rattlesnake Crotalus durissus terrificus toxins as therapeutic, theranostic and/or biotechnological agents

Revisting the potential of South American rattlesnake Crotalus durissus terrificus toxins as therapeutic, theranostic and/or biotechnological agents

Hayashi MAF, Campeiro JD, Yonamine CM. Revisting the potential of South American rattlesnake Crotalus durissus terrificus toxins as therapeutic, theranostic and/or biotechnological agents. Toxicon (2022), doi: https://doi.org/10.1016/j.toxicon.2021.12.005

A native cell-penetrating peptide (CPP) from rattlesnake with therapeutic and theranostic properties

Abstract
The cell-penetrating peptides (CPPs) are characterized by the ability of internalization into cells in vitro and in vivo, and the ability of these peptides can rely on a high content of positive charges, as it is the case of the native CPP crotamine. Crotamine is a polypeptide of about 42 amino acid residues with high content of basic residues as Arg and Lys. Although most of known CPPs are linear peptides, native the South American rattlesnake crotamine from the venom of a South American rattlesnake has a well-defined 3D structure stabilized by three disulfide bonds which guarantee the exposure of side chains of basic amino acids. This 3D structure also protects this amphipathic polypeptide from the degradation even if when administered by oral route, therefore, also protecting also the biological activities of crotamine. As several different biological properties of crotamine are dependent of cell penetration of crotamine, the methods mainly employed for analyzing crotamine properties as anthelminthic and antimalarial activities, antimicrobial and antitumor activities, with a unique selective cytotoxic property against actively proliferating cells, as tumor cells, were chosen based on crotamine its ability of internalization mediated by itsbased on positive charge. This native cationic polypeptide is also able to efficiently carry, with no need of covalent linkage with the cargo, genetic material into cells in vitroin vitro and in vivoin vivo, suggesting its use in gene therapy. Moreover, the possibility of decorating gold nanoparticles keeping the ability of transfecting cells was demonstrated. More recently, the ability of crotamine to interfere in animal metabolism, inducing browning of adipose tissue and increasing the energy expenditure, and its application in renal therapy was demonstrated. As crotamine also accumulates specifically in tumor cells in vivoin vivo, and the potential utility of crotamine as a theranostic agent was then suggested. Therefore, diverse methodologies employed for the characterization and exploration of the therapeutic applications of this promising native CPP for remediation of several pathogenic conditions are presented here.

Neuropeptidases in Psychiatric Disorders

A system biology approach based on metabolic biomarkers and protein-protein interactions for identifying pathways underlying Schizophrenia and Bipolar Disorder

Altaf-Ul-Amin M, Hirose K, Nani JV, Porta LC, Tasic L, Hossain SF, Huang M, Ono N, Hayashi MAF, Kanaya S. A system biology approach based on metabolic biomarkers and protein-protein interactions for identifying pathways underlying Schizophrenia and Bipolar Disorder. Sci Rep. 2021 Jul 14;11(1):14450. doi: 10.1038/s41598-021-93653-3.

Myotoxin a, Crotamine and Defensin Homologs in Reptile Venoms

Regulation of monoamine levels by typical and atypical antipsychotics in Caenorhabditis elegans mutant for Nuclear Distribution Element genes

D'Arc Campeiro J, Nani JV, Monte GG, Almeida PC, Mori MA, Hayashi MAF. Regulation of monoamine levels by typical and atypical antipsychotics in Caenorhabditis elegans mutant for Nuclear Distribution Element genes. Neurochem Int. 2021 Apr 17;147:105047. doi: 10.1016/j.neuint.2021.105047.

Effects of Psychostimulants and Antipsychotics on Serum Lipids in an Animal Model for Schizophrenia

Impact of Reck expression and promoter activity in neuronal in vitro differentiation

Evaluation of NDEL1 oligopeptidase activity in blood and brain in an animal model of schizophrenia: effects of psychostimulants and antipsychotics

Nani JV, Lee RS, Yonamine CM, Sant'Anna OA, Juliano MA, Gadelha A, Mari JJ, Hayashi MAF. (2020) Evaluation of NDEL1 oligopeptidase activity in blood and brain in an animal model of schizophrenia: effects of psychostimulants and antipsychotics. Sci Rep. 2020 Oct 28;10(1):18513. doi: 10.1038/s41598-020-75616-2.

Impairments in Peripheral Blood T Effector and T Regulatory Lymphocytes in Bipolar Disorder Are Associated with Staging of Illness and Anti-cytomegalovirus IgG Levels

MAES, M.. NANI, J.V., NOTO, C., RIZZO, L., HAYASHI, M. A. F., BRIETZKE, E. (2020) Impairments in Peripheral Blood T Effector and T Regulatory Lymphocytes in Bipolar Disorder Are Associated with Staging of Illness and Anti-cytomegalovirus IgG Levels. Mol Neurobiol. 2020 Jul 22:pyaa050. doi: 10.1007/s12035-020-02110-1.

Biophysical and pharmacological characterization of a full-length synthetic analog of the antitumor polypeptide crotamine

de CARVALHO PORTA, L. C., FADEL, V., CAMPEIRO, J. D., OLIVEIRA, E. B., GODINHO, R. O., & HAYASHI, M. (2020) Biophysical and pharmacological characterization of a full-length synthetic analog of the antitumor polypeptide crotamine. J Mol Med (Berl). 98(11):1561-1571. doi: 10.1007/s00109-020-01975-y.

A study in first-episode psychosis patients: does angiotensin I-converting enzyme (ACE) activity associated with genotype predict symptoms severity reductions after treatment with the atypical antipsychotic risperidone?

A study in first-episode psychosis patients: does angiotensin I-converting enzyme (ACE) activity associated with genotype predict symptoms severity reductions after treatment with the atypical antipsychotic risperidone?

NANI, J. V., DAL MAS, C., YONAMINE, C. M., OTA, V. K., NOTO, C., BELANGERO, S. I., MARI, J. J., BRESSAN, R., CORDEIRO, Q., GADELHA, A., & HAYASHI, M. (2020) A study in first-episode psychosis patients: does angiotensin I-converting enzyme (ACE) activity associated with genotype predict symptoms severity reductions after treatment with the atypical antipsychotic risperidone?. Int J Neuropsychopharmacol. 22, pyaa050. doi: 10.1093/ijnp/pyaa050.

In vivo effects of the association of the psychoactive phenotiazine thioridazine on antitumor activity and hind limb paralysis induced by the native polypeptide crotamine

PORTA, L. C., CAMPEIRO, J. D., PAPA, G. B., OLIVEIRA, E. B., GODINHO, R. O., RODRIGUES, T., & HAYASHI, M. (2020) In vivo effects of the association of the psychoactive phenotiazine thioridazine on antitumor activity and hind limb paralysis induced by the native polypeptide crotamine. Toxicon 185, 64-71. https://doi.org/10.1016/j.toxicon.2020.06.017

Crotamine Cell-Penetrating Nanocarriers: Cancer-Targeting and Potential Biotechnological and/or Medical Applications

HAYASHI, M., CAMPEIRO, J. D., PORTA, L. C., SZYCHOWSKI, B., ALVES, W. A., OLIVEIRA, E. B., KERKIS, I., DANIEL, M. C., & KARPEL, R. L. (2020) Crotamine Cell-Penetrating Nanocarriers: Cancer-Targeting and Potential Biotechnological and/or Medical Applications. Methods Mol Biol. (Clifton, N.J.) 2118, 61–89. https://doi.org/10.1007/978-1-0716-0319-2_5

Decreased nuclear distribution nudE-like 1 enzyme activity in an animal model with dysfunctional disrupted-in-schizophrenia 1 signaling featuring aberrant neurodevelopment and amphetamine-supersensitivity

Decreased nuclear distribution nudE-like 1 enzyme activity in an animal model with dysfunctional disrupted-in-schizophrenia 1 signaling featuring aberrant neurodevelopment and amphetamine-supersensitivity

NANI, J. V., FONSECA, M. C., ENGI, S. A., PERILLO, M. G., DIAS, C. S., GAZARINI, M. L., KORTH, C., CRUZ, F. C., & HAYASHI, M. A. (2020) Decreased nuclear distribution nudE-like 1 enzyme activity in an animal model with dysfunctional disrupted-in-schizophrenia 1 signaling featuring aberrant neurodevelopment and amphetamine-supersensitivity. J Psychopharmacol. (Oxford, England) 34(4), 467-477. https://doi.org/10.1177/0269881119897562

Neuropeptides and oligopeptidases in schizophrenia

Peripheral biomarkers allow differential diagnosis between schizophrenia and bipolar disorder

TASIC, L., LACERDA, A. L. T., PONTES, J. G. M., DA COSTA, T. B. B. C., NANI, J. V., MARTINS, L. G., SANTOS, L. A., NUNES, M. F. Q., ADELINO, M. P. M., PEDRINI, M., CORDEIRO, Q., DE SANTANA, F. P., POPPI, R. J., BRIETZKE, E., HAYASHI, M. A. Peripheral biomarkers allow differential diagnosis between schizophrenia and bipolar disorder. J Psychiatr Res. 2019 Dec;119:67-75. doi: 10.1016/j.jpsychires.2019.09.009. [Noticia agencia FAPESP]

Neuroinflammation and glial cell activation in mental disorders

Effects of the Natural Peptide Crotamine from a South American Rattlesnake on Candida auris, an Emergent Multidrug Antifungal Resistant Human Pathogen

DAL MAS, C., ROSSATO, L., SHIMIZU, T., OLIVEIRA, E.B., DA SILVA, J. P. I., MEIS, J. F., COLOMBO, A. L., HAYASHI, M. A. Effects of the Natural Peptide Crotamine from a South American Rattlesnake on Candida auris, an Emergent Multidrug Antifungal Resistant Human Pathogen. Biomolecules, 9(6). 2019. doi: 10.3390/biom9060205.

Animal Models in Psychiatric Disorder Studies

NANI, J. V.; RODRÍGUEZ, B.; CRUZ, F.; HAYASHI, M.A.F. Animal Models in Psychiatric Disorder Studies. In: Animal Models in Medicine and Biology, Ed. Eva Tvrdá and Sarat Chandra Yenisetti, IntechOpen, 2019. DOI: 10.5772/intechopen.89034. Available from: https://www.intechopen.com/books/animal-models-in-medicine-and-biology/animal-models-in-psychiatric-disorder-studies.

Advances and challenges in development of precision psychiatry through clinical metabolomics on mood and psychotic disorders

PEDRINI, M., CAO, B., NANI, J. V. S., CERQUEIRA, R. O., MANSUR, R. B., TASIC, L., HAYASHI, M. A. F., MCLNTYRE, R. S., BRIETZKE, E. Advances and challenges in development of precision psychiatry through clinical metabolomics on mood and psychotic disorders. Prog Neuropsychopharmacol Biol Psychiatry. 93:182-188. 2019. doi: 10.1016/j.pnpbp.2019.03.010

Ndel1 oligopeptidase activity as a potential biomarker of early stages of schizophrenia

DAL, M. C., NANI, J. V., NOTO, C., YONAMINE, C. M., DA CUNHA, G. R., MANSUR, R.B., OTA, V. K., BELANGERO, S. I., CORDEIRO, Q., KAPCZINSKI, F., BRIETZKE, E., BRESSAN, R. A., GADELHA, A., HAYASHI, M. A. F. Ndel1 oligopeptidase activity as a potential biomarker of early stages of schizophrenia. Schizophr Res. 208:202-208. 2019. doi: 10.1016/j.schres.2019.02.021.

Long term safety of targeted internalization of cell penetrating peptide crotamine into renal proximal tubular epithelial cells in vivo

CAMPEIRO, J. D., DAM, W., MONTE, G. G., PORTA, L. C., OLIVEIRA, L. C. G., NERING, M. B., VIANA, G. M., CARAPETO, F. C., OLIVEIRA, E. B., van den BORN, J., HAYASHI, M. A. F. Long term safety of targeted internalization of cell penetrating peptide crotamine into renal proximal tubular epithelial cells in vivo. Sci Rep. 9(1):3312. 2019. doi: 10.1038/s41598-019-39842-7.

ACE activity in blood and brain axis in an animal model for schizophrenia: Effects of dopaminergic manipulation with antipsychotics and psychostimulants

NANI, J. V., YONAMINE, C. M., CASTRO MUSIAL, D., DAL MAS, C., MARI, J. J., HAYASHI, M. A. F. ACE activity in blood and brain axis in an animal model for schizophrenia: Effects of dopaminergic manipulation with antipsychotics and psychostimulants. World J Biol Psychiatry. 26:1-11. 2019. doi: 10.1080/15622975.2019.1583372.

Impact of nuclear distribution element genes in the typical and atypical antipsychotics effects on nematode Caenorhabditis elegans: Putative animal model for studying the pathways correlated to schizophrenia

Impact of nuclear distribution element genes in the typical and atypical antipsychotics effects on nematode Caenorhabditis elegans: Putative animal model for studying the pathways correlated to schizophrenia

MONTE, G. G., NANI, J. V., DE ALMEIDA CAMPOS, M. R., DAL MAS, C., MARINS, L. A. N., MARTINS, L. G., TARSIC, L., MORI, M. A., HAYASHI, M. A. F. Impact of nuclear distribution element genes in the typical and atypical antipsychotics effects on nematode Caenorhabditis elegans: Putative animal model for studying the pathways correlated to schizophrenia. Prog Neuropsychopharmacol Biol Psychiatry. 92:19-30. 2019. doi: 10.1016/j.pnpbp.2018.12.010.

Insights into the Effects of Crack Abuse on the Human Metabolome Using a NMR Approach

COSTA, T. B. B. C., LACERDA, A. L. T., DAL MAS, C., BRIETZKE, E., PONTES, J. G. M., MARINS, L. A. N., MARTINS, L. G., NUNES, M. V., PEDRINI, M., CARVALHO, M. S. C., MITROVITCH, M. P., HAYASHI, M. A. F., SALDANHA, N. L., POPPI, R. J., TARSIC, L. Insights into the Effects of Crack Abuse on the Human Metabolome Using a NMR Approach. J Proteome Res. 18(1):341-348. 2019. doi: 10.1021/acs.jproteome.8b00646.

Oligopeptidases activity in bipolar disorder: Ndel1 and angiotensin I converting enzyme

Pharmacological characterization of crotamine effects on mice hind limb paralysis employing both ex vivo and in vivo assays: Insights into the involvement of voltage-gated ion channels in the crotamine action on skeletal muscles

Pharmacological characterization of crotamine effects on mice hind limb paralysis employing both ex vivo and in vivo assays: Insights into the involvement of voltage-gated ion channels in the crotamine action on skeletal muscles

LIMA, S. C., PORTA, L. C., LIMA, Á. D. C., CAMPEIRO, J. D., MEURER, Y., TEIXEIRA, N. B., DUARTE, T., OLIVEIRA, E. B., PICOLO, G., GODINHO, R. O., SILVA, R. H., HAYASHI, M. A. F. Pharmacological characterization of crotamine effects on mice hind limb paralysis employing both ex vivo and in vivo assays: Insights into the involvement of voltage-gated ion channels in the crotamine action on skeletal muscles. PLoS Negl Trop Dis. 12(8):e0006700. 2018. doi: 10.1371/journal.pntd.0006700.

Crotamine induces browning of adipose tissue and increases energy expenditure in mice

Design and characterization of crotamine-functionalized gold nanoparticles

KARPEL, R. L., DA SILVA LIBERATO, M., CAMPEIRO, J. D., BERGEON, L., SZYCHOWSKI, B., BUTLER, A., MARINO, G., CUSIC, J. F., DE OLIVEIRA, L. C. G., OLIVEIRA, E. B., DE FARIAS, M. A., PORTUGAL, R. V., ALVES, W. A., DANIEL, M. C., HAYASHI, M. A. F. Design and characterization of crotamine-functionalized gold nanoparticles. Colloids Surf B Biointerfaces. 163:1-8. 2018. doi: 10.1016/j.colsurfb.2017.12.013.

Oral treatment with a rattlesnake native polypeptide crotamine efficiently inhibits the tumor growth with no potential toxicity for the host animal and with suggestive positive effects on animal metabolic profile

Oral treatment with a rattlesnake native polypeptide crotamine efficiently inhibits the tumor growth with no potential toxicity for the host animal and with suggestive positive effects on animal metabolic profile

CAMPEIRO, J. D., MARINOVIC, M. P., CARAPETO, F. C., DAL MAS, C., MONTE, G. G., PORTA, L. C., NERING, M. B., OLIVEIRA, E. B., HAYASHI, M. A. F. Oral treatment with a rattlesnake native polypeptide crotamine efficiently inhibits the tumor growth with no potential toxicity for the host animal and with suggestive positive effects on animal metabolic profile. Amino Acids. 50(2):267-278. 2018. doi: 10.1007/s00726-017-2513-3.

A preliminary study of bipolar disorder type I by mass spectrometry-based serum lipidomics

RIBEIRO, H. C., KLASSEN, A., PEDRINI, M., CARVALHO, M. S., RIZZO, L. B., NOTO, M. N., ZENI-GRAIFF, M., SETHI, S., FONSECA, F. A. H., TASIC, L., HAYASHI, M. A. F., CORDEIRO, Q., BRIETZKE, E., SUSSULINI, A. A preliminary study of bipolar disorder type I by mass spectrometry-based serum lipidomics. Psychiatry Res. 258:268-273. 2017. doi: 10.1016/j.psychres.2017.08.039.

Biophysical and biological properties of small linear peptides derived from crotamine, a cationic antimicrobial/antitumoral toxin with cell penetrating and cargo delivery abilities

Dal Mas C, Pinheiro DA, Campeiro JD, Mattei B, Oliveira V, Oliveira EB, Miranda A, Perez KR, Hayashi MAF. Biophysical and biological properties of small linear peptides derived from crotamine, a cationic antimicrobial/antitumoral toxin with cell penetrating and cargo delivery abilities. Biochim Biophys Acta Biomembr. 2017 Dec;1859(12):2340-2349. doi: 10.1016/j.bbamem.2017.09.006.

Microbiota abnormalities and the therapeutic potential of probiotics in the treatment of mood disorders

RIOS, A. C., MAURYA, P. K., PEDRINI, M., ZENI-GRAIFF, M., ASEVEDO, E., MANSUR, R. B., WIECK, A., GRASSI-OLIVEIRA, R., MCLNTYRE, R. S., HAYASHI, M. A. F., BRIETZKE, E. Microbiota abnormalities and the therapeutic potential of probiotics in the treatment of mood disorders. Rev Neurosci. 28(7):739-749. 2017. doi: 10.1515/revneuro-2017-0001.

¹H-NMR, ¹H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling

SETHI, S., PEDRINI, M., RIZZO, L. B., ZENI-GRAIFF, M., MAS, C. D., CASSINELLI, A. C., NOTO, M. N., ASEVEDO, E., CORDEIRO, Q., PONTES, J. G. M., BRASIL, A. J. M., LACERDA, A., HAYASHI, M. A. F., POPPI, R., TASIC, L., BRIETZKE, E. ¹H-NMR, ¹H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling. Int J Bipolar Disord. 5(1):23. 2017. doi: 10.1186/s40345-017-0088-2.

Lipidomics, Biomarkers, and Schizophrenia: A Current Perspective

Metabolomics and lipidomics analyses by 1H nuclear magnetic resonance of schizophrenia patient serum reveal potential peripheral biomarkers for diagnosis

TASIC, L., PONTES, J. G. M., CARVALHO, M. S., CRUZ, G., DAL MAS, C., SETHI, S., PEDRINI, M., RIZZO, L. B., ZENI-GRAIFF, M., ASEVEDO, E., LACERDA, A. L. T., BRESSAN, R. A., POPPI, R. J., BRIETZKE, E., HAYASHI, M. A. F. Metabolomics and lipidomics analyses by 1H nuclear magnetic resonance of schizophrenia patient serum reveal potential peripheral biomarkers for diagnosis. Schizophr Res. 185:182-189. 2017. doi: 10.1016/j.schres.2016.12.024.

NDE1 and NDEL1 from genes to (mal)functions: parallel but distinct roles impacting on neurodevelopmental disorders and psychiatric illness

A current snapshot of common genomic variants contribution in psychiatric disorders

Genome-wide investigation of schizophrenia associated plasma Ndel1 enzyme activity

GADELHA, A., COLEMAN, J., BREEN, G., MAZZOTI, D. R., YONAMINE, C. M., PELLEGRINO, R., OTA, V. K., BELANGERO, S. I., GLESSNER, J., SLEIMAN, P., HAKONARSON, H., HAYASHI, M. A. F., BRESSAN, R. A. Genome-wide investigation of schizophrenia associated plasma Ndel1 enzyme activity. Schizophr Res. 172(1-3):60-7. 2016. doi: 10.1016/j.schres.2016.01.043.

Inhibition of malaria parasite Plasmodium falciparum development by crotamine, a cell penetrating peptide from the snake venom

El Chamy Maluf S, Dal Mas C, Oliveira EB, Melo PM, Carmona AK, Gazarini ML, Hayashi MA. Inhibition of malaria parasite Plasmodium falciparum development by crotamine, a cell penetrating peptide from the snake venom. Peptides. 2016 Apr;78:11-6. doi: 10.1016/j.peptides.2016.01.013.

Crotamine: Anthelmintic effects of a cationic toxin from a South American rattlesnake venom

Convergent evidences from human and animal studies implicate angiotensin I-converting enzyme activity in cognitive performance in schizophrenia

Gadelha A, Vendramini AM, Yonamine CM, Nering M, Berberian A, Suiama MA, Oliveira V, Lima-Landman MT, Breen G, Bressan RA, Abílio V, Hayashi MA. Convergent evidences from human and animal studies implicate angiotensin I-converting enzyme activity in cognitive performance in schizophrenia. Transl Psychiatry. 2015 Dec 8;5(12):e691. doi: 10.1038/tp.2015.181.